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010-62817090Assay Biotechnology 重組蛋白和合成肽
Assay Biotechnolog公司自2006年1月成立以來,Assay Biotechnology一直是業(yè)界領 xian 的抗體生產(chǎn)和工程和測定技術,熒光染料,猝滅劑,重組蛋白和合成肽的全球貢獻者。位于加利福尼亞州舊金山灣區(qū)的心臟地帶。
在我們成立后的頭幾年,Assay Biotech成立了抗體制造商和抗體工程公司,通過OEM合作伙伴關系專門針對大型公司。從那時起,我們一直在成為一個突出的測定和抗體來源,將其覆蓋范圍擴大到全球超過50個國家的數(shù)百萬研究人員。
我們一直在尋求推動我們潛力的信條,決心成為業(yè)界的測定開發(fā)和制造公司之一,提供大量即用型測定,免疫試劑,分子檢測模塊,生物標志物和全線抗體工程服務支持zui艱巨的學術,藥學和臨床研究工作。
協(xié)議:
將所有試劑制備至工作濃度,將標準品制備至所需范圍,并將樣品制備至適當?shù)南♂屢蜃印?/span>
取出所需數(shù)量的捕獲抗體包被條進行實驗,并將剩余的條帶放回裝有干燥劑的干燥袋中,進行 4°C 儲存。
向每個孔中加入100μl標準品/樣品,并在室溫(RT)下在軌道振蕩器上孵育2小時。
吸出溶液,向每個正在使用的孔中加入300μl1x洗滌緩沖液,并在軌道振蕩器上輕輕振蕩2-3分鐘。重復此過程 3 次。最后一次洗滌后,通過倒置盤子并將其敲擊在干凈的紙巾上,確保沒有液體殘留。
向每個孔中加入 100 μl 1x 檢測抗體并孵育 2 小時。在 RT 的軌道振動臺上
重復步驟 4。
向每個孔中加入100μl1x SAV-HRP,并在室溫軌道振蕩器上孵育30分鐘
重復步驟 4。
向每個孔中加入100μl復合增強劑/過氧化物溶液,蓋板免受光照,并在室溫軌道振蕩器上孵育5分鐘。
在光度計上讀取板。
基于比色的細胞ELISA
基于比色的基于細胞的 ELISA 試劑盒可檢測各種靶蛋白以及某些刺激條件對不同細胞系中靶蛋白表達的影響。通過間接ELISA形式實現(xiàn)目標蛋白濃度的定性測定。
從本質上講,靶蛋白被靶標特異性一抗 (1°) 捕獲,而 HRP 偶聯(lián)的二抗 (2°) 結合 1° 抗體的 Fc 區(qū)域。通過這種結合,與 2° 抗體偶聯(lián)的 HRP 酶可以在添加底物時催化比色反應。
由于基于細胞的 ELISA 的定性性質,描述了多種歸一化方法:1) 包括對人 GAPDH 具有特異性的單克隆抗體,作為歸一化目標吸光度值的內部陽性對照。2)通過添加底物對HRP活性進行比色測量后,采用結晶紫全細胞染色法測定細胞密度。
染色后,可以通過將吸光度值歸一化為細胞量來分析結果,從而可以調整鍍層差異。3) 如果檢測到磷酸化靶標,將提供針對非磷酸化對應物的抗體用于標準化目的。獲得的非磷酸化靶標的吸光度值可用于歸一化磷酸化靶標的吸光度值。
下面是Assay Biotechnology公司的部分熱銷產(chǎn)品
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
Tryptophan Hydroxylase (Phospho-Ser260) Antibody | P12-1295-100 | 100 ug |
HSL (Phospho-Ser855/554) Antibody | A0074-50 | 50 ug |
PFKFB2 (Phospho-Ser483) Antibody | A0761-50 | 50 ug |
eNOS (Phospho-Ser615) Antibody | A1145-50 | 50 ug |
PYK2 (Phospho-Tyr402) Antibody | A7204-50 | 50 ug |
SREBP-1 (Phospho-Ser439) Antibody | A8425-50 | 50 ug |
SP1 Antibody | B0577-50 | 50 ug |
Tryptophan Hydroxylase Antibody | B1011-50 | 50 ug |
IRS-1 Antibody | B7120-50 | 50 ug |
Paxillin Antibody | B8250-50 | 50 ug |
OCT2 Antibody | C0282-50 | 50 ug |
HARS Antibody | C10349-50 | 50 ug |
CGK 2 Antibody | C10961-50 | 50 ug |
IRX2 Antibody | C11651-50 | 50 ug |
Collagen IX alpha3 Antibody | C12212-50 | 50 ug |
ABHD12 Antibody | C14209-50 | 50 ug |
ST6GAL1 Antibody | C15170-50 | 50 ug |
AASDHPPT Antibody | C16447-50 | 50 ug |
APOBEC3D/F Antibody | C17872-50 | 50 ug |
SFRS15 Antibody | C18932-50 | 50 ug |
THY1 Antibody | C21278-50 | 50 ug |
ITGAD Antibody | C30328-50 | 50 ug |
SAMHD1 Antibody | C30986-50 | 50 ug |
GNRHR Antibody | G276-50 | 50 ug |
OR6K2 Antibody | G663-50 | 50 ug |
Caspase 6 (Cleaved-Asp162) Antibody | L0106-50 | 50 ug |
S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody | P12-1174-50 | 50 ug |
Lyn Antibody | R12-2230-50 | 50 ug |
ANGPTL7 Antibody | R12-2486-50 | 50 ug |
EIF4E3 Antibody | R12-2742-50 | 50 ug |
MARK3 Antibody | R12-2998-50 | 50 ug |
OR6P1 Antibody | R12-3254-50 | 50 ug |
SIRT3 Antibody | R12-3510-50 | 50 ug |
eEF2K (Phospho-Ser366) Antibody | A0071-100 | 100 ug |
14-3-3 thet/tau (Phospho-Ser232) Antibody | A0758-100 | 100 ug |
Catenin-alpha1 (Phospho-Ser641) Antibody | A1140-100 | 100 ug |
PTEN (Phospho-Ser370) Antibody | A7201-100 | 100 ug |
Gab2 (Phospho-Ser623) Antibody | A8350-100 | 100 ug |
p70 S6 Kinase Antibody | B0534-100 | 100 ug |
HER3 Antibody | B0946-100 | 100 ug |
FKHRL1 Antibody | B7087-100 | 100 ug |
RSK1/2/3/4 Antibody | B8182-100 | 100 ug |
Keratin 10 Antibody | C0249-100 | 100 ug |
MOS Antibody | C10258-100 | 100 ug |
Mevalonate Kinase Antibody | C10868-100 | 100 ug |
TREF1 Antibody | C11526-100 | 100 ug |
CDC5L Antibody | C12171-100 | 100 ug |
PRKAG1 Antibody | C14134-100 | 100 ug |
CBLN2 Antibody | C15057-100 | 100 ug |
GBP3 Antibody | C16080-100 | 100 ug |
4E-BP1 Monoclonal Antibody | N0001 | 50ul |
A1BG Monoclonal Antibody | N0004 | 50ul |
AATM Monoclonal Antibody | N0006 | 50ul |
AIF-M1 Monoclonal Antibody | N0017 | 50ul |
YB1 (Phospho-Ser102) Antibody | P12-1200 | 50ul |
Tryptophan Hydroxylase (Phospho-Ser260) Antibody | P12-1195 | 50ul |
TIF-IA (Phospho-Ser649) Antibody | P12-1189 | 50ul |
STMN1 (Phospho-Ser62) Antibody | P12-1182 | 50ul |
MAP2K7 (Phospho-Thr275) Antibody | P12-1140 | 50ul |
LIMK2 (Phospho-Thr505) Antibody | P12-1137 | 50ul |
GRK2 (Phospho-Ser685) Antibody | P12-1112 | 50ul |
FLT3 (Phospho-Tyr969) Antibody | P12-1108 | 50ul |
FAK (Phospho-Tyr397) Antibody | P12-1103 | 50ul |
B-RAF (Phospho-Ser446) Antibody | P12-1080 | 50ul |
ATF1 (Phospho-Ser63) Antibody | P12-1077 | 50ul |
AhR (Phospho-Ser36) Antibody | P12-1072 | 50ul |
ATP-citrate synthase (Phospho-Ser455) Antibody | P12-1003 | 50ul |
ACK (Phospho-Tyr518) Antibody | P12-1001 | 50ul |
EGFR (Phospho-Thr693) Antibody | A0009 | 50ul |
HSP90β (Phospho-Ser254) Antibody | A0013 | 50ul |
ALK (Phospho-Tyr1586) Antibody | P12-1002 | 50ul |
Adducin α/β (Phospho-Ser726/713) Antibody | A0002 | 50ul |
GSK3α/β (Phospho-Tyr279/216) Antibody | A0012 | 50ul |
C/EBP α (Phospho-Ser21) Antibody | A0056 | 50ul |
Caspase-9 (Phospho-Thr125) Antibody | A0060 | 50ul |
eEF2K (Phospho-Ser366) Antibody | A0071 | 50ul |
Cyclin D1 (Phospho-Thr286) Antibody | A0417 | 50ul |
Goat Anti-Chicken IgY (H&L) - Cy7 | SA1257 | 50ul |
Goat Anti-Human IgG gamma chain - Cy7 | SA1261 | 50ul |
Goat Anti-Human IgM mu chain - Cy7 | SA1263 | 100 ul |
Goat Anti-Rabbit IgG (H&L) - Cy7 | SA1267 | 100 ul |
Rabbit Anti-Chicken IgY (H&L) - Cy7 | SA1270 | 100 ul |
Rabbit Anti-Guinea Pig IgG (H&L) - Cy7 | SA1275 | 100 ul |
Mouse Anti-Human Ig lambda chain - Cy7 | SA1297 | 100 ul |
Rabbit Anti-Human IgG F(ab')2 - Cy7 | SA1299 | 100 ul |
Rabbit Anti-Human IgG (H&L) - Cy7 | SA1277 | 100 ul |
Rabbit Anti-Human IgG gamma chain - Cy7 | SA1279 | 100 ul |
Rabbit Anti-Monkey IgG (H&L) - Cy7 | SA1282 | 100 ul |
Goat Anti-Chicken IgY (H&L) - AMCA | SA0440 | 100 ul |
Goat Anti-Human IgG Fab - AMCA | SA0442 | 100 ul |
Goat Anti-Human IgG+IgM+IgA - AMCA | SA0445 | 100 ul |
Goat Anti-Rabbit IgG (H&L) - AMCA | SA0450 | 100 ul |
Goat Anti Chicken IgY (H&L) - Alexa Fluor 750 | SA1042 | 100 ul |
Goat Anti Rabbit IgG (H&L) - Alexa Fluor 750 | SA1052 | 100 ul |
Rabbit Anti Dog IgG (H&L) - Alexa Fluor 750 | SA1056 | 100 ul |
Rabbit Anti Guinea Pig IgG (H&L) - Alexa Fluor 750 | SA1060 | 100 ul |
Rabbit Anti Human IgG Fc - Alexa Fluor 750 | SA1063 | 100 ul |
Rabbit Anti Human IgG+IgM+IgA - Alexa Fluor 750 | SA1065 | 100 ul |
Rabbit Anti Monkey IgG (H&L) - Alexa Fluor 750 | SA1067 | 100 ul |
Rabbit Anti Sheep IgG (H&L) - Alexa Fluor 750 | SA1071 | 100 ul |
Mouse Anti Human IgG Fab - Alexa Fluor 750 | SA1075 | 100 ul |
Mouse Anti Human IgM - Alexa Fluor 750 | SA1077 | 100 ul |
Mouse Anti Human Ig kappa chain - Alexa Fluor 750 | SA1081 | 100 ul |
BMAL1 (Acetyl-Lys538) Colorimetric Cell-Based ELISA Kit | CBA1086 | 1 Kit |
p73 (Acetyl-Lys327) Colorimetric Cell-Based ELISA Kit | CBA1085 | 1 Kit |
13269198101
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